P-87: High Fat Diet Affects In Vitro Maturation and In Vitro Fertilization of Mouse Oocytes
نویسندگان
چکیده مقاله:
Background: Physiological effects of obesity are increasingly common in women of reproductive age and are associated with infertility. Obese women have dysfunction in hypothalamic-pituitary-ovarian axis, menstrual cycle and are up to three times more likely to have decreased oligo-/anovulation. The purpose of present study was to evaluate the high fat diet on IVM and IVF outcomes in mouse. Materials and Methods: Balb-c mice (21 days of age, n=40) were divided into control and high fat diet groups. Experimental group fed with high fat diet (60% fat) during 12 weeks. After 12 weeks the oocytes obtained and in vitro maturation (IVM) and in vitro fertilization (IVF) were done according to routine protocols. Results: The results showed significantly decrease in number of oocytes obtained at GV stage in high fat diet group and had weaker expansion as well as fewer oocytes reached to metaphase II stage. IVF results showed that number of MII oocytes in HFD group decreased significantly compared to control group and 30% of them reached to two-cell embryo. Conclusion: Our Findings show that high fat diet causes a significant reduction in differentiated oocytes from GV stage to methaphase II and also reduce the number of two-cell embryos in balb -c mices. It is suggested that in obese women with high body mass index maturation of oocyte and pregnancy rate is lower than normal ones.
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عنوان ژورنال
دوره 7 شماره 3
صفحات 70- 70
تاریخ انتشار 2013-09-01
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